A Bioorthogonal Double Fluorogenic Probe to Visualize Protein–DNA Interaction

Two sets of bioorthogonally applicable, double fluorogenic probes, capable sensing DNA–protein interactions, were prepared by installing an azide or tetrazine motif onto structurally fluorogenic, DNA sensitive frames. Installation these bioorthogonal functions intercalating dyes furnished scaffolds with reactivity based fluorogenicity, rendering probes double-fluorogenic, AND-type logic switches that require the simultaneous occurrence a reaction and interaction to trigger high intensity fluorescence. The evaluated for behavior in presence/absence complementary function. Our studies revealed appending thiazole orange frames show remarkable features. One membrane permeable modified derivative was further tested live cell labeling studies. Cells expressing bioorthogonalized DNA-binding proteins showed intensive fluorescence characteristics localization upon treatment our probe. On contrary, similarly cytosolic did not result appearance signal. These suggest such double-fluorogenic are indeed interactions cells.